Buch

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Übersicht

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Verlag	:	Dr. Hut
Buchreihe	:	Mikrosystemtechnik
Sprache	:	Englisch
Erschienen	:	24. 02. 2025
Seiten	:	224
Einband	:	Kartoniert
Höhe	:	240 mm
Breite	:	170 mm
Gewicht	:	423 g
ISBN	:	9783843955713
Sprache	:	Englisch

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Produktinformation

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Porous materials are an extremely technologically attractive and unique class of materials for interaction with low abundance biomarkers due to their highly specific surfaces. When the high porosity of a material is coupled with functionalization for specific interaction with molecules in a penetrating fluid, porous materials can present a powerful tool for many applications in bioanalytics. In this work, two design philosophies were combined and utilized for the development of porous materials: precise pore definition and high surface-to-volume ratio. In the former case, nickel bioaffinity membranes fabricated via a combination of photolithography and electrochemistry with specified pore sizes and locations were developed for CTC isolation. The membranes were primed with a phosphonic acid to add C,H groups for surface attachment of a polystyrene layer via the C,H insertion crosslinking (CHic) reaction with a thermal crosslinker (PS-co-MAz) followed by a layer of PDMAA-co-MABP. EpCAM antibodies were added via streptavidin-biotin bridges for specific interaction with CTCs. Demonstration of the CTC isolation capabilities at very low cell counts (< 10 cells) of MCF-7 cells (as model CTCs) in 7.5 ml and 50 ml of whole blood indicated nearly perfect capture efficiencies. In the second scenario, paper-like fiber mats were fabricated via electrospinning, producing core-sheath fibers with non-swelling cores from PMMA-co-MABP and hydrogel sheaths from PDMAA-co-MABP. The polymers utilized included CHic crosslinker groups (MABP), allowing for improvements to the fiber thermal and solvent stability, and wetting properties. This material was then used in a lateral flow assay for borreliosis with OspC and Bb VlsE1 as the biomarkers, and fluorescence as the readout. Signal-to-background ratios of 4:1 could be achieved. This material was then adapted for a CTC capture by functionalizing with anti-EpCAM, exhibiting high potential as an isolation membrane.

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